Comparison of the content of active compounds in cell suspensions and aqueous extracts of Rosmarinus officinalis L. with those in the Endophytic fungus Alternaria alternata

Authors

  • Rabab Abed University of Diyala Author
  • Batoul Mohammed Alwan University of Diyala Author

DOI:

https://doi.org/10.69923/y7d55k89

Keywords:

Endophytic Fungi, Rosmarinus officinalis ,cell suspension

Abstract

The current study was carried out to detect the active compounds in both the aqueous extract of a Rosmarinus officinalis L. plant and the extract of cell suspensions and compare them with the extract of the endophytic fungus Alternaria alternata isolated from the leaves of an R. officinalis plant. Callus was induced from the leaves of rosemary plants, and the highest induction of callus formed reached 100% when treated with a concentration of 1-Naphthaleneacetic Acid (NAA) 2.0 mg. L-1, compared to the control treatment, which had an induction rate of 0%. The highest fresh weight of the callus was 1.628 gm in the presence of NAA. The callus induced in the presence of NAA was characterized by its fragile texture. Cell suspensions were also obtained from it. The fungus extract was superior in its content of active compounds to the aqueous extract and cell suspension cultures at 21 days. The total alkaloid content was 6.65%, phenolic content was 117.25 mg. gm, flavonoid content was 104.35 mg. gm, total terpenoid content was 6.65%, total steroid content was 6.05%, and the concentration of rosemary acid was 154.7%. The results of the current study confirm the single receptor of endophytic fungi isolated from medicinal plants as one of the important sources of effective compounds.

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Author Biographies

  • Rabab Abed, University of Diyala

    Dr. Rabab Majead Abed Lecturer in the Department of Biology - College of Education for Pure Sciences- Diyala University, Iraq. Bachelor's degree from University of Diyala , and Master's degree from University of Diyala, PhD degree was obtained from the University of Baghdad. Her research areas are mycology and branches related to this science, such as plant diseases, biocontrol, biofertilization, and medical mycology.She can be contacted at email: rabab.abed@uodiyala.edu.iq.

     

  • Batoul Mohammed Alwan, University of Diyala

    Batoul Mohammed Alwan : PhD student in Department of Biology - College of Education for Pure Sciences - University of Diyala. She can be contacted via email: sadeh1970@gmail.com.

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Published

09/30/2024

Issue

Vol. 1 No. 2 (2024)

Section

Articles

License

Copyright (c) 2024 Iraqi Journal for Applied Sciences

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References

Marshall,G.C.and H.W.Ohm 1987 .Yield responses of 16 winter wheat cultivars to row spacing and seeding rate .Agronomy Journal .79:1027-1030

How to Cite

[1]
“Comparison of the content of active compounds in cell suspensions and aqueous extracts of Rosmarinus officinalis L. with those in the Endophytic fungus Alternaria alternata”, IJApSc, vol. 1, no. 2, pp. 93–101, Sep. 2024, doi: 10.69923/y7d55k89.

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