Statistical optimization of Lipase yield using Aspergillus sydowii BTSS 1005 a novel isolate identified from a marine sediment at East Coast of Visakhapatnam

Abstract

Lipase, a versatile biocatalyst, has many biotechnological applications. A lipolytic fungal strain, BTSS 1005, was isolated from a marine sediment on the eastern coast of Visakhapatnam. After sequencing studies of my previous work, it was identified as a novel strain of Aspergillus sydowii. It was found to produce a thermostable lipase by submerged fermentation during my research and hence was selected for bioprocess development. In the present work, media composition was optimized for the enhancement of lipase activity using statistical techniques. The major ingredients were chosen through Plackett-Burman experimental design, and the optimum ingredient level was investigated through Central Composite Design, of Response Surface Methodology, for best lipase yield. The activity of lipase produced by submerged fermentation studies, initially determined as 28.64 U/ml before optimization, was predicted 63.94 U/ml by using RSM. This study was useful for paving the way for the usage of different statistical models in the optimization of cultural parameters in industrial productions of bioactive compounds.