In vitro morphogenesis and conservation of the medicinal plant Vitex negundo L. through nodal explants

Dr Subba Tata Sape; P. Sudhakara Rao   Rao

doi:10.69923/bnnavj49

Authors

Dr Subba Tata Sape Department of Biotechnology, Andhra University Author
P. Sudhakara Rao Andhra University Author

DOI:

https://doi.org/10.69923/bnnavj49

Keywords:

Direct Organogenesis MS medium Nodal Explant Plant growth regulators Rhizogenesis

Abstract

Vitex negundo L. is an important medicinal plant widely used in traditional medicine and is valued for its diverse pharmacological properties. The present study was undertaken to develop an efficient in vitro regeneration and multiplication protocol for V. negundo using nodal explants and to establish a suitable approach for ex situ conservation. Nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins: 6-benzylaminopurine (BAP), kinetin (KIN), and thidiazuron (TDZ), either individually or in combination with auxin indole-3-acetic acid (IAA). Among the cytokinins tested, TDZ was the most effective for shoot induction. MS medium containing 2.0 mg L⁻¹ TDZ produced 92.6% shoot induction, with an average of 10.2 shoots per explant. Further optimization using auxin–cytokinin combinations demonstrated that MS medium supplemented with 2.0 mg L⁻¹ TDZ and 0.5 mg L⁻¹ IAA was highly effective, producing up to 96.3% shoot induction and 26.6 shoots per explant. The regenerated shoots were subjected to root on half-strength MS media containing IBA. The highest rooting response was obtained on half-strength MS medium containing 0.75 mg L⁻¹ IBA, with 100% root induction, 12.8 roots per shoot, and a mean root length of 4.9 cm. The rooted plantlets were successfully hardened and acclimatized, with 100% survival reported under field conditions. The developed protocol provides an efficient method for rapid multiplication and ex situ conservation of V. negundo and may facilitate the production of quality planting material for medicinal plant cultivation.

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Author Biographies

Dr Subba Tata Sape, Department of Biotechnology, Andhra University

Dr. S. Subba Tata is an assistant professor at the College of Science and Technology, Andhra University, Andhra Pradesh, India. He received the B.Sc. degree in CBZ, the M.Sc. degree in Biotechnology, the M.Tech. degree in Biotechnology, and the M.Sc. degree in Botany from Andhra University. He holds a Ph.D. degree in plant sciences with a plant tissue culture specialization. He qualified for the CSIR, APSET, ASRB-NET & GATE competitive examinations. His research areas are plant genetic engineering and molecular genetics. He has published several scientific papers in national and international conferences and journals. He can be contacted at E-mail: drsubbatata@andhrauniversity.edu.in
P. Sudhakara Rao , Andhra University

Dr. P. Sudhakara Rao is an assistant professor at the College of Science and Technology, Andhra University, Andhra Pradesh, India. He received his B.Sc. and M.Sc. degrees in biotechnology from Andhra University. He holds a Ph.D. degree in plant sciences with a plant tissue culture specialization. His research areas are plant tissue culture and photochemistry. He has published several scientific papers in national and international conferences and journals. He can be contacted at E-mail: sudhakar@andhrauniversity.edu.in

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